Rapid urea broth test for yeasts.
نویسندگان
چکیده
A rapid, miniaturized, urea broth test useful for detecting urease activity of yeasts was compared to Christensen urea agar. All urease-producing yeasts tested were positive on both media; however, 60% were reactive in the urea R broth within 30 min, and the remainder were reactive within 4 h. This urea multiwell test may be useful as a rapid screening method for detecting urease-producing yeasts recovered from clinical specimens and as an adjunct test with other rapid methods of yeast identification.
منابع مشابه
Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients.
The presence of C pylori infection was determined in 1445 patients undergoing upper gastrointestinal endoscopy over a 12 month period. The presence of C pylori was detected in gastric mucosal biopsy specimens by the biopsy urease test, microscopy (Gram stained smears and histology) and culture. Two media were used for the biopsy urease test: Christensen's urea broth (for the first 600 patients)...
متن کاملRapid method for detection of urea hydrolysis by yeasts.
A method is described for the rapid detection of urea hydrolysis by yeasts, using the Berthelot color reaction. The results could be determined within 30 to 50 min with this method, compared with 8 to 72 h usually required with Christensen urea agar.
متن کاملRapid biochemical screening for Salmonella, Shigella, Yersinia, and Aeromonas isolates from stool specimens.
Four screens for the rapid (4 to 6 h) biochemical detection of pathogens from enteric isolation media are described. The Salmonella screen consisted of Kligler iron agar (KIA), motility-indole-urea-tryptophan-deamination semisolid medium (MIU-TDA), and the o-nitrophenyl-beta-D-galactopyranoside (ONPG) test; the Shigella screen consisted of KIA, MIU-TDA, the ONPG test, and the lysine decarboxyla...
متن کاملRapid method for testing raffinose fermentation by yeasts.
The alcoholic fermentation of raffinose by yeasts has considerable taxonomic importance. Certain species do not ferment this trisaccharide at all; others ferment only the fructose part; whereas others ferment both parts (fructose and melibiose). All or some strains of a few species ferment melibiose incompletely. Most older methods for testing raffinose fermentation are based on quantitative de...
متن کاملUse of a urease test for the screening and identification of cryptococci.
The increase in mycological work by diagnostic laboratories has stimulated the development of simple and reliable procedures for routine screening and identification of fungus cultures. The writer has investigated the possibility of applying procedures for the diagnosis of yeasts other than the widely used sugar fermentation and assimilation tests. During earlier investigations it was found tha...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 7 6 شماره
صفحات -
تاریخ انتشار 1978